To observe the continued network-based transaction between a callback domain and the malware running on an enterprise PC while reducing the risk of lateral spread and the risk that the adversary would notice any changes, the best technique to use is to emulate the malware in a heavily monitored DMZ segment. This is a secure environment that is isolated from the rest of the network and can be heavily monitored to detect any suspicious activity. By emulating the malware in this environment, the activity can be observed without the risk of lateral spread or detection by the adversary. References:
https://www.sans.org/blog/incident-response-fundamentals-why-is-the-dmz-so-important/